金属蛋白質の研究(第10報) : 各種金属およびアミノ酸のコハク酸酸化酵素系に対する影響

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The effects of amino acid chelate on succinoxidase activity were studied and the inhibitory action of cupric ion on oxidation of succinate by mouse liver homogenate was compared with that of amino acid chelates of copper. Of nine amino acid chelates examined, methionine, lysine, and especially histidine chelate showed marked inhibitory effect on succinoxidase than cupric ion alone (Fig.1 and Table II). In these investigations, the effect of addition of various metals was examined and it was shown that the addition of Mn^<2+> as well as Ca^<2+> and Al^<2+> to succinoxidase system accelerated the rate of oxygen uptake (Table III). With increasing concentration of Mn^<2+>, increase of succinate oxidation was observed but inhibition occurred with further increase of Mn^<2+> (10^<-3>M) (Fig.2). Addition of Mn^<2+> and histidine markedly accelerated the aerobic oxidation of succinate and it was found that the effect of this acceleration was not due to chelate formation (Fig.3). Oxidation of succinate in succinoxidase system was strongly inhibited by oxalacetic acid, but the activity was restored by the addition of Mn^<2+> and histidine (Fig.4).
公益社団法人日本薬学会の論文
1966-08-25