芍薬エキス中のPaeoniflorinの高速液体クロマトグラフィーによる定量

概要

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A method was established for the quantitative determination of paeoniflorin in Paeony extract by high performance liquid chromatography. Paeoniflorin can be separated using a μ-Bondapak C_<18> column with a mobile phase (CH_3CN : H_2O : CH_3COOH=15 : 85 : 1) at ambient. Paeony extract contains paeoniflorin as the main ingredient, and albiflorin, oxypaeoniflorin and benzoylpaeoniflorin. Paeoniflorin cannot be determined exactly by the gas liquid chromatography method by separating it from other sub-ingredients. While high performance liquid chromatography methods, such as the absolute and the gradient method, have been reported for the determination of paeoniflorin, comparison of our method with others revealed that the present method is superior due to simplicity, analytical time and accuracy.
社団法人日本薬学会の論文
1979-06-25