蛋白の高速液体クロマトグラフィー(第3報)逆相高速液体クロマトグラフィーによる蛋白の分離

概要

論文の詳細を見る
Conventionally, in gel permeation chromatography (GPC) it has been almost impossible to separate and identify proteins having near molecular weight. As for high performance liquid chromatography (HPLC) method, which has been developed in our previous report, it was examined whether it can be applied to simultaneous, efficient separation and identification of six kinds of proteins having molecular weight of 21000-26000 ; trypsin inhibitor (soy beans), trypsin, trypsinogen, α-chymotrypsin, α-chymotrypsinogen and elastase. Under isocratic elution system by using Nucleosil CN as a stationary phase and sodium ethanesulfonate, sodium pentanesulfonate or octanesulfonate as an ion-pair reagent in mobile phase, all of them could be satisfactorily chromatographed.
社団法人日本薬学会の論文
1981-08-25