^3H標識Mannanの生体内運命(第2報)組織中のMannanの免疫学的検討

概要

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The structual integrity of radioactive substances in various tissues of mouse recovered after the administration of labeled baker's yeast mannan was examined by the immunological method. 1) Antiserum was prepared from rabbits immunized with the heat-killed whole cells of Saccharomyces cerevisiae. The agglutinin titer of the antiserum was in the range of 1 : 1280 to 1 : 2560 against the living cells of S. cerevisiae. 2) Upon quantitative analysis of the precipitin reaction of ^3H-labeled mannan with the antiserum, 50% of the radioactivity was precipitated in the case of antibody excess and equivalence zone. 3) The radioactive substances in the reticuloendothelial tissues of the mouse after injection of ^3H- or ^<14>C-mannan had a precipitability similar to mannan for 1 week, and the activity decreased progressively, but dissapeared rapidly from the kidney.
社団法人日本薬学会の論文
1974-11-25