シリカゲルを担体とする水性二相液体クロマトグラフィーシステムにおける薬物およびその代謝物の保持挙動

概要

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The retention indices of 17 drugs and metabolites including hypnotics, antiepileptics, analgesics, and central nervous system stimulants in liquid-liquid chromatography were systematically examined to devise a highly efficient extraction and fractionation procedure in biological fluid analysis. A high performance system incorporating an acid-treated silica gel column and a UV-detector was assembled to determine the capacity ratios in aqueous two phase organic solvents consisting of diethyl ether and n-hexane (1 : 0,7 : 3,1 : 1,3 : 7 v/v). The corrected capacity ratios (k'_<cor>) of the drugs in an aqueous organic phase system were calculated from the experimental retention data and phase ratios in liquid-liquid chromatography. A linear correlation between the logarithm of the k'_<cor> value and the logarithm of solvent composition in a liquid-liquid partition system was demonstrated in this study.
公益社団法人日本薬学会の論文
1983-02-25