水性二成分系溶媒を用いるシリカゲル液体クロマトグラフィーにおける脂溶性生体成分の保持挙動

概要

論文の詳細を見る
A high performance liquid-liquid partition chromatographic system involving two immiscible liquid phases, water and a binary organic solvent, was made from a slurry packed silica gel column, operated by a droplet current to pump the organic solvent equilibrated at the same time as the water and a ultraviolet detector provided with a flow-type cell. The binary organic solvent, water saturated and containing diethyl ether or ethanol in n-hexane as a phase system was used for the measurement of the retention indices of lipophilic components in biological substances such as cholesterol esters, lipophilic vitamins and steroid hormones. The molecular structure of each solute was found to be correlated to its respective retention index by a linear relation between the logarithm of the capacity ratio and that of solvent composition in the mobile phase solvent. The retention data thus obtained were directly related to the liquid-liquid distribution coefficient and thus applicable to making the better selection of an appropriate optimum phase system for use in solvent extraction processes.
公益社団法人日本薬学会の論文
1989-09-25