生体試料の前処置法としての水性液・液分配カラムによる抽出過程の高効率化

概要

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An improved column fractionation procedure was developed for the analysis of biological samples. According to the simulation of the solute distribution in two phases for various extraction models, a multi-step liquid-liquid partition technique employing packed columns was found more effective than the usual funnel separation-batch method and the droplet current distribution procedure. As an alternative to the open-bed extraction column used for the pre-treatment of the biological fluids, a closed-bed column system was introduced through the incorporation of a ultraviolet monitor. Drugs and their metabolites in urine were extracted quantitatively using diethyl ether as a carrier solvent. The aqueous liquid-liquid distribution column having a high theoretical plate number was prepared using diatomaceous earth consisting of small size particles and applying a slurry packing procedure.
公益社団法人日本薬学会の論文
1982-11-25