迅速流れ分画法とHPLC法の組合せによる腎臓移植患者の血清コルチゾール測定

概要

論文の詳細を見る
Serum cortisol level of kidney transplant patients treated with prednisolone as an immunosuppressive agent was detertnined by a combination of rapid-flow fractionation (RFF) and high performance liquid chromatography (HPLC). A 0.5ml serum was extracted with ether using RFF technique prior to HPLC to obtain cortisol fraction. The ether extract was analyzed by HPLC with normal phase silica gel column and organic solvent system as a mobile phase solvent. Detection limit of this method was as low as 500pg/ml of serum. The cortisol peak was not disturbed by concomitant drugs such as azathioprine or cyclosporin A.
日本医療薬学会の論文
1988-12-20