中性子放射化分析による生体中微量元素の研究(第4報)正常ラットおよびカドミウム投与ラット肝臓可溶性蛋白の結合金属元素について

概要

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Cytosol proteins of rat liver were divided into four fractions by gel filtration on Sephadex G-75 column. Neutron activation analysis was carried out to determine 7 elements (Mn, Cu, Cd, Fe, Co, Zn and Se) in each protein. Fe, Mn and Se were mainly combined with large molecular weight proteins, while Cd, Cu and Zn with low molecular weight proteins. It was recognized that Zn quantity in low molecular weight fraction was increased significantly by Cd injection. The behavior of Zn in the fraction was examined by use of ^<65>Zn tracer. Although Zn-thionein was not produced after administration of small amount (0.2 mg) of Zn, it was observed by the injection of 1 mg of Zn. The effect of Cd on Zn elution profile was studied. Cd-thionein was induced by Cd injection, and then Zn content in metallothionein fraction increased. From a result of distribution of Zn among organs, it could be presumed that the increase of Zn was based on renal Zn. Further, metallothionein previously produced by Cd injection was combined with about 28% of Zn incorporated into the liver.
社団法人日本薬学会の論文
1981-06-25