In VitroおよびIn Vivoにおけるラット肝臓メタロチオネインとカドミウム, 銅, 亜鉛の結合について

概要

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Rat liver metallothioneins (MT) induced by 6 times injection (s.c.) of Cd, Cu or Zn (0.1 mg at each time) were isolated by Sephadex G-75 gel filtration. The metals were removed from MTs by treating them with EDTA. The thionein (t-MT) of MT treated with EDTA or Cd-MT was incubated with 2.5μmol each of Cd, Cu and Zn per mg of protein. The binding of these three metals to t-MT or Cd-MT was investigated in vitro. Further, the in vivo incorporation of each metal into hepatic MT of rats was also studied by injecting rats with 0.1 mg of Cd 6 times and then reinjecting (s.c.) with 10μmol each of Cd, Cu and Zn 24 hours after the final injection of Cd. MT was bound up to 8-9 g atom metals per mol of protein in vitro. The decreasing order of affinity of MT is considered to be Cu>Zn>Cd. The substitution of metals for the Cd-MT was observed. On the other hand, the amount of bound metals in the MT was increased by reinjection of each metal and the increments were based on the reinjected metals. The metals incorporated later are considered to be trapped by the present MT.
社団法人日本薬学会の論文
1982-06-30