Concentration and Degradation of Hyaluronic Acid in Knee Synovial Fluid from Carrageenin-Induced Rabbit Arthritis
スポンサーリンク
概要
- 論文の詳細を見る
The concentration and degradation of hyaluronic acid in the synovial fluid of carrageenin-induced arthritic joints of rabbits was studied. A 0.5-ml volume of 1% λ-carrageenin was intra-articularly injected three times into a right knee joint, and saline into a left. After 5 d from the last injection, inflammatory changes were observed in the synovial membrane and synovial fluid, but not in the articular cartilage. In the inflammatory synovial fluid, lipid peroxide content, phosphatase activity and cell counts were significantly increased, but the copper concentration was not changed. Concentration of polymeric hyaluronic acid and total hyaluronic acid were determined by high-performance liquid chromatography using gel-permeation columns. Total hyaluronic acid was appreciably decreased in the inflammatory fluid. The polymeric hyaluronic acid determined was 38% of the toal hyaluronic acid in the inflammatory fluid and 74% in the control fluid. This suggests that in the inflammatory fluid, molecular weights of hyaluronic acid are distributed in the broader range. The concentration of chondroitin sulphates was similar in both the inflammatory fluid and the control fluid, but the content ratio of chondroitin sulphates to hyaluronic acid was higher in the inflammatory fluid. In the inflamed synovial membrane, synthesis of hyaluronic acid as measured by incorporation of [^<14>C]glucosamine into glycoconjugates was increased by about twice that in the control membrane.
- 社団法人日本薬学会の論文
- 1990-07-25
著者
-
森 五彦
Department Of Radiopharmaceutical Chemistry Kobe Women's College Of Pharmacy
-
森 五彦
神戸薬科大
-
本橋 範子
Department of Radiopharmaceutical Chemistry, Kobe Women's College of Pharmacy
-
中道 良好
Department of Pharmaceutical Radiochemistry, Kobe Women's College of Pharmacy
-
西川 均
Oyodo Research Laboratory, Maruho Company, Ltd.,
-
梅本 準治
Oyodo Research Laboratory, Maruho Company, Ltd.,
-
本橋 範子
Department Of Radiopharmaceutical Chemistry Kobe Women's College Of Pharmacy
-
中道 良好
Department Of Pharmaceutical Radiochemistry Kobe Women's College Of Pharmacy
-
西川 均
Oyodo Research Laboratory Maruho Company Ltd.
-
梅本 準治
Oyodo Research Laboratory Maruho Company Ltd.
-
中道 良好
Department of Hygienic Sciences, Kobe Women's College of Pharmacy
関連論文
- 脾臓の免疫機能イメ-ジング剤としてのプトレシンの有用性〔英文〕
- 腫瘍細胞移植によるマウス脾臓中のポリアミン含量及びその代謝関連酵素活性について
- In VitroおよびIn Vivoにおけるラット肝臓メタロチオネインとカドミウム, 銅, 亜鉛の結合について
- ラット肝臓中のメタロチオネイン生成における誘導金属量について
- The Concentrations of the Bound Metals in Hepatic Metallothionein of Rats injected with Cadmium
- 中性子放射化分析による生体中微量元素の研究(第4報)正常ラットおよびカドミウム投与ラット肝臓可溶性蛋白の結合金属元素について
- The Study of Elements in Organisms by Neutron Activation Analysis. III. The Distribution of Trace Elements in Various Organs of Normal Rat and in Cell Fractions of Rat Liver
- 中性子放射化分析による大麻葉の多種類元素分析 : 国内産大麻の産地別比較
- 中性子放射化分析による生体中微量元素の研究(第2報)栽培大麻の各部位における無機元素の分布について
- 中性子放射化分析法による大麻中のリンの定量-^Pの液体シンチレーションスペクトロメーターによるチェレンコフ光測定-
- Isolation and Characterization of α-Mercaptopropionylglycine-2,4-Dihydroxyphenylalanine Conjugates Produced through Mushroom Tyrosinase Reaction
- Inhibitory Effects of Sulfur Compounds on Melanin Formation Reaction by Tyrosinase
- Concentration and Degradation of Hyaluronic Acid in Knee Synovial Fluid from Carrageenin-Induced Rabbit Arthritis
- Quantitation of Hyaluronic Acid and Chondroitin Sulphates in Rabbit Synovial Fluid by High-Performance Liquid Chromatography of Oligosaccharides Enzymatically Derived Thereof
- The Role of Ergothioneine in the Oxidation of Reduced Nicotinamide Adenine Dinucleotide by Metmyoglobin or Methemoglobin
- Radioassay for Oxidation of Tyrosine by Tyrosinase using L-[Ring-^3H] tyrosine and L-[Carboxyl-^C] tyrosine
- L-リジンおよびポリ-L-リジンの57Coによる標識反応とそれらの正常およびエ-ルリッヒ担がんマウスにおける体内分布
- Distribution of Some Disulfhydryl-Containing Chelating Agents labeled with Indium-113m and Gallium-67 in Mice
- Radioprotective Effect of Ergothioneine on γ-Irradiation of Metmyoglobin : Comparison with Cysteine on Sulfmyoglobin-Formation
- RI取扱者としての基礎知識は充分条件でない(薬学におけるFIの教育と研究の課題)
- Complexing of Copper Ion by Ergothioneine
- ^13C-Nuclear Magnetic Resonance and Raman Spectroscopic Studies on Ionization and Mercury Complex of Ergothioneine
- Metal Complexes of Ergothioneine
- 82. 我教室の精製Gonadotropinと米国NIH並びにKoln大学のGonadotropinとの電気泳動学的比較検討について
- 16. 豚下垂体前葉よりのGonadotropin並びに尿中Gonadotropinの連続濾紙電気永動法による分離と糖成分について
- 薬学と放射性医薬品
- 有害性重金属水銀(II),銅(II),鉛(II),カドミウム(II),ビスマス(III)の電気泳動による迅速分離ならびに定量 : 多そう式電気泳動法による研究(第3報)
- pHこう配利用による指示薬試薬の焦点ゾーン電気泳動法 : 多そう式電気泳動法による研究(第2報)
- 7 電気泳動法
- 薄層クロマトシート電気泳動法による90Sr-90Y,212Pb-212Biの迅速分離
- Lack of evidence for existence of vitamin D and 25-hydroxyvitamin D sulfates in human breast and cow's milk.
- ペーパークロマトグラフィーによる上昇展開中のアルカリ金属イオンに対する電示的定量
- ペーパークロマトグラフィーにおける展開中の無機イオンに対する直流加電圧検出装置の試作