エイコサペンタエン酸及びアラキドン酸によるマウス腹腔細胞 : 特に多形核白血球の誘導とその性質(生物学及び生化学)

概要

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Eicosapentaenoic acid (EPA) was injected into the peritoneal cavity of mice, ICR-male mice 6-8 weeks old, and larger numbers of polymorph nuclear leucocytes (PMN, 2-3×10^7 cells/mouse) were obtained compared with other irritants (thioglycolate and zymosan). From a normal mouse peritoneal cavity, about 2-3×10^6 cells were collected; populations were : 1.3-1.9×10^6 macrophages, 0.6-0.9×10^6 lymphocytes and 1.4-2.1×10^5 PMNs. Into the peritoneal cavity of mice one of three irritants in the following amounts was injected : 1 ml 3% thioglycolate, 200 mg of zymosan or 0.05 ml of EPA. Then after intervals of 15,40 or 60 h collected peritoneal exudate cells were counted and stained by using the Wright-Giemsa methods. In EPA groups, after 40 h the percentage of PMNs to total cells were 80-85%, cell numbers changed 1.4-2.1×10^5 PMNs in normal to 2-3×10^7 PMNs. Very similar results were obtained when arachidonic acid was injected into mouse peritoneal cavities. EPA- or arachidonic acid-treated PMNs were analyzed for lipid composition. At 24 h after EPA injection, PMNs contained about 20-30% of EPA in lipid fractions. The amount of EPA in phospholipids' fraction changed similarly to that in total lipids. O_2^- release from EPA-rich PMNs decreased more than that from thioglycolate treated cells. Phagocytotic activity decreased about 50% compared with thioglycolate-treated PMNs.
公益社団法人日本薬学会の論文
1986-11-25