マウス腹腔マクロファージ調製時の物理的刺激と6-ケトーブロスタグランジンF_<1α>およびトロンボキサンB_2産生について

概要

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The contents of 6-keto-prostaglandin F_<1α> (6KF_<1α>) and thromboxane B_2 (TXB_2) in mouse peritoneal macrophages were determined by radioimmunoassay. When indomethacin at a final concentration of 0.1 mM was added at each stage in the preparation of macrophage samples, i.e. (I) before incubation of peritoneal exudate cells in glass dishes to prepare macrophage monolayers, (II) before harvest of macrophages from the glass surfaces, (III) before sonication of macrophage suspensions, and (IV) before centrifugation of sonicated macrophage solutions, large differences were detected in the contents of 6KF_<1α> and TXB_2 at each stage. These results suggested that physical stimulation during the preparation of samples resulted in increases of 6KF_<1α> and TXB_2 production by macrophages.
社団法人日本薬学会の論文
1982-08-25