各種クロラミンによるポリオウイルスの不活化

概要

論文の詳細を見る
Inactivation rates for poliovirus (Lsc, 2ab strain), suspended in a phosphate buffer salt solution (PBS) and a cell maintenance medium (MEM), with chloramines, N-chlorosuccinimide (Cl-SI), chloramine T (Cl-T), ammoniamonochloramine (NH_2Cl), N-chlorodiethylamine (ClDEA) and N-chloroglycine (Cl-Gly) at 2 and 10 mg/l as available chlorine (Cl_2), were determined at pH 7 and 20℃. Inactivation efficacies of these chloramines were compared each other and discussed in relation to chemical potency, hydrolysis constants and chlorination rates for the chloramines. At 10 mg/1 as Cl_2,the periods for the 99% inactivation of the virus suspended in PBS with Cl-SI, Cl-T, and NH_2Cl were 2.5,5,and 5 min, respectively. However, Cl-DEA and Cl-Gly did not reach 99% inactivation in 10 min contact. There were a little chlorine consumption in the test solutions of Cl-SI, Cl-T, and NH_2Cl but of Cl-DEA and Cl-Gly. The inactivation efficacies of these chloramines did not correspond to the order of their hydrolysis constants. The faster chlorination rate for Cl-SI than tested other chloramines may have an influence on its faster inactivation rate. Inactivation rates for the virus suspended in MEM with the chloramines were slower than that for the virus suspended in PBS. It might be due to the formation of less effective chloramines from constituents in MEM virus suspension.
社団法人日本薬学会の論文
1997-08-31