A New Assay for Evaluating Hepatotoxicity and Cytotoxicity Using LDL-Uptake Activity of Liver Cells

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概要

• 論文の詳細を見る

• A rapid and sensitive bioassay for detecting cytotoxicity was developed in this study to be used in evaluating many kind of chemicals. This assay, based on the LDL (low density lipoprotein)-uptake activity of human hepatoblastoma cells, Hep G2,can evaluate cytotoxicity for 48h with high sensitivity and selectivity using a 96 well plate and a fluorescent plate reader. We evaluate the toxicity of 230 kinds of chemicals and formulate the dose response data by a simple mathematical equation. The toxicity parameters derived by the formulation had some correlations in terms of chemical groups, which were classified as aromatics, organics, metals, and so on.

• 公益社団法人日本薬学会の論文
• 2000-12-01

著者

• Utsumi Hideo

  Faculty of Pharmaceutical Sciences, Kyushu University

• Sakai Yasuyuki

  Institute of Industrial Science, University of Tokyo

• SUZUKI MOTOYUKI

  Institute of Industrial Science, University of Tokyo

• Suzuki M

  Institute Of Industrial Science University Of Tokyo

• SAKODA Akiyoshi

  Institute of Industrial Science, University of Tokyo

• Shoji Ryo

  Department of Chemical Science and Engineering, Tokyo National College of Technology

• SHOJI RYO

  Institute of Industrial Science, University of Tokyo

• Shoji Ryo

  Institute Of Industrial Science University Of Tokyo

• Shoji Ryo

  Department Of Chemical Science And Engineering Tokyo National College Of Technology

• Sakai Y

  Center For Disease Biology And Integrative Medicine Graduate School Of Medicine University Of Tokyo

• Suzuki Motoyuki

  Institute Of Technology And Science The University Of Tokushima

• Sakoda A

  Institute Of Industrial Science University Of Tokyo

• Sakoda Akiyoshi

  Institute Of Industrial Science University Of Tokyo

• Sakai Yasuyuki

  Institute Of Industrial Science (iis) The University Of Tokyo

• Utsumi Hideo

  Faculty Of Pharmaceutical Sciences Kyushu University

• Utsumi Hideo

  Faculty Of Pharmaceutical Science Kyushu University

• Suzuki Motoyuki

  Institute Of Industrial Science University Of Tokyo

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