Cloning and Expression of the Isopenicillin N Synthase Gene from Lysobacter lactamgenus YK90 : A portion of this work was presented at the 6th International Symposium on Genetics of Industrial Microorganisms (GIM90)
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概要
- 論文の詳細を見る
The Lysobacter lactamgenus YK90 pcbC gene encoding isopenicillin N synthase (IPNS) was cloned using the corresponding Acremonium chrysogenum pcbC gene as a probe. The cloned pcbC gene encoded a polypeptide composed of 326 amino acid residues with a molecular weight of 36,562. The predicted amino acid sequence had a high similarity with those from fungus A. chrysogenum and actinomycetes Streptomyces clavuligerus. The pcbC gene was expressed in E. coli and in A. chrysogenum under control of the tac and GAP promoters, respectively.
- 社団法人日本生物工学会の論文
- 1995-08-25
著者
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Kimura Hiroyuki
Fermentation Center Technology Development Laboratories Takeda Chemical Industires Ltd. :(present Ad
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Suzuki M
Institute Of Industrial Science University Of Tokyo
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SUZUKI MASARU
Fermentation Center, Technology Development Laboratories, Takeda Chemical Industries, Ltd.,
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SUMINO YASUHIRO
Fermentation Center, Technology Development Laboratories, Takeda Chemical Industries, Ltd.,
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Sumino Y
Takeda Chemical Ind. Ltd. Osaka Jpn
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Sumino Yasuhiro
Fermentation Center Technology Development Laboratories Takeda Chemical Industries Ltd.
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Suzuki M
Kikkoman Corp. Chiba Jpn
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