Sequencing of the β-Isopropylmalate Dehydrogenase Gene (LEU2) from Acremonium chrysogenum and Its Application to Heterologous Gene Expression
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概要
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The nucleotide sequence of the Acremonium chrysogenum β-isopropylmalate dehydrogenase gene (LEU2) was determined. The LEU2 gene encoded a polypeptide composed of 380 amino acid residues with a molecular weight of 40,637 Da. The predicted amino acid sequence had a high degree of similarity (47%) with that of the Saccharomyces cerevisiae LEU2 gene. A 1.4 kb transcript, which was repressed in the presence of leucine, was detected. A fused gene, LEU2-HPT, consisting of the LEU2 promoter and the open reading frame of the bacterial hygromycin B phosphotransferase (HPT) gene was constructed and could be used as a dominant selectable marker in an A. chrysogenum transformation system. Moreover, the Escherichia coli β-galactosidase gene (lacZ) was expressed under the control of the LEU2 promoter in A. chrysogenum.
- 社団法人日本生物工学会の論文
- 1995-12-25
著者
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Kimura Hiroyuki
Fermentation Center Technology Development Laboratories Takeda Chemical Industires Ltd. :(present Ad
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Suzuki M
Institute Of Industrial Science University Of Tokyo
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MATUMURA SHIGEO
Fermentation Center, Technology Development Laboratories, Takeda Chemical Industries, Ltd.,
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SUZUKI MASARU
Fermentation Center, Technology Development Laboratories, Takeda Chemical Industries, Ltd.,
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SUMINO YASUHIRO
Fermentation Center, Technology Development Laboratories, Takeda Chemical Industries, Ltd.,
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Sumino Y
Takeda Chemical Ind. Ltd. Osaka Jpn
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Sumino Yasuhiro
Fermentation Center Technology Development Laboratories Takeda Chemical Industries Ltd.
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Matumura Shigeo
Fermentation Center Technology Development Laboratories Takeda Chemical Industries Ltd. :(present Ad
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Suzuki M
Kikkoman Corp. Chiba Jpn
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