ATTACHMENT KINETICS OF ANIMAL CELLS IMMEDIATELY AFTER CONTACT ONTO SPECIFIC AND/OR NON-SPECIFIC SURFACES

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概要

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• Attachment kinetics of BHK cells in very short contact times (0, 1, 2, 3 and 5 min) under static conditions was investigated on tissue-culture-treated polystyrene (TC-PS) dishes preadsorbed with various proteins. Although cells attached rapidly onto bare TC-PS, they did not attach at all within 1–2 min of contact when the surfaces were preadsorbed with serum albumin (Alb), even at low surface coverage. The specific interaction between adsorbed fibronectin (FN) and cells required a short contact time to accomplish the attachment. Moreover, FN adsorption at low surface coverage retarded the cell attachment up to 2 min as compared to the bare TC-PS. These inhibitory effects of FN were completely overcome by postcoating with polylysine (PL), which did not inhibit the spreading promoting activity of FN. Both Alb and FN adsorption had some contributions in enhancing the surface hydrophobicity, whereas post-treatment of the FN adsorbed surfaces by PL did not change the characteristics at all. Having considered that Alb and FN themselves are negatively charged at physiological pH, cell contact and attachment initiation onto solid surfaces once preadsorbed with proteins were strongly suggested to be predominated by electrostatic interactions simply by evaluating the charge sign of adsorbed proteins.

• 社団法人 化学工学会の論文

著者

• Suzuki M

  Institute Of Industrial Science University Of Tokyo

• ISAKAI Yasuyuki

  Institute of Industrial Science, University of Tokyo

• ISAKODA Akiyoshi

  Institute of Industrial Science, University of Tokyo

• SUZUKI Motoyuk

  Institute of Industrial Science, University of Tokyo

• Isakai Yasuyuki

  Institute Of Industrial Science University Of Tokyo

• Suzuki Motoyuki

  Institute Of Technology And Science The University Of Tokushima

• Isakoda Akiyoshi

  Institute Of Industrial Science University Of Tokyo

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