Specific Amplification of NADH Using NADH Kinase in a Reaction Mixture Containing Excess NAD^+
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概要
- 論文の詳細を見る
NADH kinase exists in yeast mitochondria and phosphorylates NADH specifically. We obtained NADH kinase having high specificity and stability sufficient for the practical use from the aerobic yeast Pichia membranaefaciens. By using this enzyme, we succeeded in amplification of NADH without the influence of co-existing NAD^+, by phosphorylating NADH into NADPH and putting this NADPH through an NADP^+ -NADPH cycling system.
- 社団法人日本農芸化学会の論文
- 1994-05-23
著者
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Ohno T
Noda Institute For Scientific Research
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Horiuchi Tatsuo
Research And Development Division Kikkoman Corporation
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Horiuchi Tatsuo
Research And Development Division Of Kikkoman Corporation
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SUZUKI MASARU
Research and Development Division, Kikkoman Corporation
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Suzuki M
Institute Of Industrial Science University Of Tokyo
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Suzuki M
Kikkoman Corp. Chiba Jpn
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Ohno Tsuyoshi
Noda Institute for Scientific Research
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Suzuki Masaru
Research & Development Division Kikkoman Corporation
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