A NEW METHOD FOR PERMEABILIZATION OF CULTURED CELLS WITHOUT CELL DAMAGE
スポンサーリンク
概要
- 論文の詳細を見る
Transient permeabilization was readily achieved by vortex-stirring cultured mammalian cells for a few seconds in the presence of 1 to 10 μg/ml of high molecular weight polyacrylic acid (PAA). This was demonstrated by an appreciable uptake by murine leukemia L1210 cells of Lucifer Yellow (LY), which is known to be a non-permeant except under conditions in which micropores are induced in the plasma membrane. In addition, the cytotoxicity of bleomycin (BLM), a very poor permeant, was markedly enhanced by vortex-stirring with PAA. Scope and limitation of this permeabilization procedure is preliminarily described.
- 公益社団法人日本薬学会の論文
- 1996-03-15
著者
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KAWAZOE Yutaka
Faculty of Pharmaceutical Sciences, Nagoya City University
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Kawazoe Yutaka
Faculty Of Pharmaceutical Sciences Nagoya City University
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Kawazoe Yutaka
Faculty Of Pharmaceutical Science University Of Tokyo
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Kawazoe Y
Nagoya City Univ. Nagoya Jpn
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SHIMIZU Naoaki
Faculty of Pharmaceutical Sciences, Nagoya City University
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Shimizu Naoaki
Faculty Of Pharmaceutical Sciences Nagoya City University
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