DELICATE AND CRUCIAL PH DEPENDENCE IN PERMEABILIZATION OF CULTURED MAMMALIAN CELLS BY VORTEX-STIRRING WITH A HIGH MOLECULAR WEIGHT POLYACRYLIC ACID
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概要
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We previously reported that cultured mammalian cells are effectively permeabilized by vortex-stirring the cells with a high molecular weight polyacrylic acid. The present study revealed that the efficiency of permeabilization of a non-permeant dye, Lucifer Yellow (LY), was sensitively affected by the pH of the medium. When the cells are vortex-stirred in RPMI 1640 culture medium, the pH of the medium should be adjusted with HEPES and NaOH, in stead of NaHCO_3,to a pH higher than 7.6 for successful permeabilization; the higher the pH, the better the result obtained. Internalization of LY was near the background level at a pH below 7.4. When phosphate-buffered saline was substituted for RPMI 1640 medium, the optimal pH range was slightly shifted to a more acidic region. This requirement for the pH of the medium is indispensable as a supplement to the standard permeabilization procedure tentatively proposed in the preceding report : Biol. Pharm. Bull., 19,1279-1284 (1996).
- 公益社団法人日本薬学会の論文
- 1996-11-15
著者
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KAWAZOE Yutaka
Faculty of Pharmaceutical Sciences, Nagoya City University
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Kawazoe Yutaka
Faculty Of Pharmaceutical Science University Of Tokyo
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SHIMIZU Naoki
Faculty of Pharmaceutical Sciences, Nagoya City University
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Shimizu Naoaki
Faculty Of Pharmaceutical Sciences Nagoya City University
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Shimizu Naoki
Faculty Of Pharmaceutical Sciences Nagoya City University
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