A New Method for Permeabilization of the Plasma Membrane of Cultured Mammalian Cells. VII. Permeabilization of Growing and Resting Cells by Vortex-Stirring with High Molecular Weight Polyacrylic Acid
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概要
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The permeability of murine leukemia L1210 cells toward non-permeant fluorescent Lucifer Yellow (LY) was evaluated by flow cytometry following vortex-stirring of cells with LY in the presence of high molecular weight polyacrylic acid (A-119). Permeabilization by this technique was greatly enhanced in growth phase cells, whereas it was appreciably reduced in resting cells which had been maintained in unchanged culture medium for 2 to 4 d.This reduction in permeabilization of resting cells gradually disappeared when resting cells were re-suspended in fresh culture medium, and the degree of permeabilization was restored to that of the growing cells. It is recommended that the mammalian cells used for internalization of non-permeant materials by vortex-stirring with A-119 should be in the early log phase.
- 公益社団法人日本薬学会の論文
- 1996-12-15
著者
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KAWAZOE Yutaka
Faculty of Pharmaceutical Sciences, Nagoya City University
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Kawazoe Yutaka
Faculty Of Pharmaceutical Science University Of Tokyo
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Noda Yasuhiro
Faculty Of Pharmaceutical Sciences Nagoya City University
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SHIMIZU Naoaki
Faculty of Pharmaceutical Sciences, Nagoya City University
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Shimizu Naoaki
Faculty Of Pharmaceutical Sciences Nagoya City University
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