A New Method for Permeabilization of the Plasma Membrane of Cultured Mammalian Cells. II. Mechanism for Potentiation of Bleomycin Cytotoxicity by Vortex-Stirring with High Molecular Weight Polyacrylic Acid
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概要
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As previously reported, the cytotoxicity of bleomycin (BLM) toward cultured mammalian cells was remarkably potentiated by vortex-stirring this agent with the cells for a few seconds in the presence of high molecular weight polyacrylic acid (PAA). This paper describes that the enhancement of BLM cytotoxicity was also achieved by a sequential BLM-treatment immediately after vortex-stirring cells with PAA only. It was suggested that this enhancement resulted from the transient permeabilization of the plasma membrane induced during vortex-stirring in the presence of PAA, leading to an increase in BLM incorporation. This mechanism was supported by the finding of an appreciable uptake by vortex-stirred cells of Lucifer Yellow, a known non-permeant material. This procedure, which did not affect any cell viability, might be a beneficial method for transient permeabilization of cultured mammalian cells to facilitate the internalization of non-permeant materials.
- 公益社団法人日本薬学会の論文
- 1996-06-15
著者
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KAWAZOE Yutaka
Faculty of Pharmaceutical Sciences, Nagoya City University
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Kawazoe Yutaka
Faculty Of Pharmaceutical Science University Of Tokyo
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Kawazoe Y
Nagoya City Univ. Nagoya Jpn
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SHIMIZU Naoki
Faculty of Pharmaceutical Sciences, Nagoya City University
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Shimizu Naoki
Faculty Of Pharmaceutical Sciences Nagoya City University
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