Improvement of Fusing Ability of Human Sperm to Zona-Free Hamster Eggs by Conditioned Media

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概要

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• A conditioned medium (CM) was prepared by culturing human sperm at 3.0×10^6 sperm/ml for 12 h. The fusion index increased significantly when the sperm penetration assay (SPA) was performed with CM (0.6 without CM to 2.0 with CM). The conditioned medium was also effective on the sperm from 11 patients who showed a low or zero value in SPA and who's in vitro fertilization treatment failed. When CM from the sperm of healthy donors was added to these patients'sperm, the fusing ability to zona free hamster eggs was significantly restored.

• 社団法人日本薬学会の論文
• 1995-01-15

著者

• 田中 慶一

  大阪大学 大学院薬学研究科毒性学分野

• 田中 慶一

  Faculty of Nutrition , Kobe-Gakuin University

• 伊川 正人

  JSPS Research Fellow Osaka University

• 應 星

  Faculty of Pharmaceutical Sciences Osaka University

• 大橋 一友

  Osaka University Faculty of Medicine Osaka University

• 佐治 文隆

  Osaka University Faculty of Medicine Osaka University

• 岡部 勝

  The Research Institute for Microbial Diseases Osaka University

• 田中 慶一

  大阪大学大学院薬学研究科

• Okabe Masaru

  The Research Institute For Microbial Diseases Osaka University

• 田中 慶一

  大阪大学大学院 薬学研究科 毒性学分野

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