Effects of Ryanodine and Nifedipine on Contractions Induced by Norepinephrine, Acetylcholine, and Potassium in the Isolated Vas Deferens of the Guinea Pig

スポンサーリンク

概要

• 論文の詳細を見る

• In the present study we tried to determine the relative contributions of sarcoplasmic reticulum (SR) calcium release and gated calcium entry through voltage-dependent calcium-channels (VDC) to the contractions induced by norepinephrine (NE, 10 μM) and acetylcholine (ACh, 10 μM) in the vas deferens of the guinea pig. NE and ACh at 10μM caused contractions composed of three phases : a prephasic, a phasic and a tonic component. Nifedipine strongly inhibited the contractions induced by potassium (K, 100mM) and abolished phasic components of the contractions induced by NE or ACh. However, the tonic components of NE- or ACh-induced contractions were slightly attenuated by nifedipine. Pretreatment with ryanodine in combination with caffeine attenuated prephasic and tonic components of the contractions induced by NE and ACh in a dose-dependent manner. The inhibitory effects of ryanodine and caffeine on the phasic component of NE-induced contractions were divided into two types : 1) abolishment of the phasic contractions and 2) a delayed appearance of the phasic contractions without attenuation of magnitude. However, ryanodine with caffeine minimally affected phasic component by ACh-and K-induced contractions. Ryanodine pretreatment in combination with NE, ACh or K had no effect on the contractions induced by each agonist. These results suggest that 1) SR Ca release is essential for the initiation and/or the maintenance of the tonic components of NE- or ACh-induced contractions as well as for the initiation of the prephasic components, 2) the phasic components by NE depend on both the SR Ca release and gated Ca entry but those induced by ACh depend only on gated Ca entry, 3) the Ca-induced Ca-release mechanism may be scarcely involved in SR Ca release induced by NE and ACh.

• 公益社団法人日本薬学会の論文

著者

• GOMI YASUO

  Department of Pharmacology, Faculty of Pharmaceutical Science, Kanazawa University

• Gomi Yasuo

  Department Of Pharmacology Faculty Of Pharmaceutical Science Kanazawa University

• TSUNOBUCHI Hiromi

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kanazawa University

• Tsunobuchi Hiromi

  Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kanazawa University

関連論文

• PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. VII. INHIBITORY EFFECT OF DIBENAMINE ON COCAINE-INDUCED SUPERSENSITIVITY OF ISOLATED VAS DEFERENS OF GUINEA PIG
• Effects of Mazindol on the Contractions in the Rat Vas Deferens
• Spontaneous Tone in Different Types of Longitudinal Muscle Preparations of Guinea Pig Ileum
• STUDIES ON ISOLATED SMOOTH MUSCLE CELLS.VIII.^Ca-EFFLUX FROM SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM TAENIA COLI OF GUINEA PIG
• STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. IV. ISOLATION AND ACETYLCHOLINE-CONTRACTION OF SINGLE SMOOTH MUSCLE CELLS FROM TAENIA COLI OF GUINEA-PIG
• EFFECTS OF 1-(2-CHLORO-4-HYDROXYPHENYL)-t-BUTYL-AMINOETHANOL (HOKU-81), A NEW BRONCHODILATOR, ON ISOLATED TRACHEA AND ATRIA OF GUINEA PIG
• PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. VIII. DISSOCIATION OF INDUCTION OF SUPERSENSITIVITY FROM PRESYNAPTIC ACTION OF COCAINE ON ISOLATED VAS DEFERENS OF GUINEA PIG
• STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. VII. RESPONSE TO AGONISTS AND THE CONTRACTION VELOCITY OF TAENIA COLI OF GUINEA PIG AND THE SINGLE SMOOTH MUSCLE CELLS
• PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. XII. INHIBITORY EFFECT OF RESERPINE ON TRIPELENNAMINE-, N, N-DIMETHYL-N', N'-DIBENZYLEHYLENEDIAMINE- AND N, N-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE-INDUCED SUPERSENSITIVITY OF ISOLATED VAS DEFERENS OF G
• PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. XI. INHIBITORY EFFECT OF DIBENAMINE ON TRIPELENNAMINE-N, N-DIMETHYL-N', N'-DIBENZYLETHYLENEDIAMINE- AND N, N'-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE-INDUCED SUPERSENSITIVITY OF ISOLATED VAS DEFERENS OF G
• PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. X.EFFECT OF TRIPELENNAMINE, N, N-DIMETHYL-N', N'-DIBENZYLETHYLENEDIAMINE AND N, N-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE ON THE UTILIZATION OF CALCIUM IN ACETYLCHOLINE-INDUCED CONTRACTION OF ISOLATED VAS
• PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. IX. NONSPECIFIC SUPERSENSITIVITY OF VAS DEFERENS OF GUINEA PIG INDUCED BY TRIPELENNAMINE, N, N-DIMETHYL-N', N'-DIBENZYLETHYLENEDIAMINE AND N, N-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE
• Propranolol Blocks Cocaine-Induced Potentiation of the Contraction in the Smooth Muscle of the Rat Vas Deferens
• The Uptake of Manganese Induced by Agonists in the Isolated Vas Deferens of the Guinea Pig
• Effects of Ryanodine and Nifedipine on Contractions Induced by Norepinephrine, Acetylcholine, and Potassium in the Isolated Vas Deferens of the Guinea Pig
• Tachyphylaxis to the Inhibitory Effect of Manganese on Potassium-Induced Contractions in the Isolated Vas Deferens of the Guinea Pig
• PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. VI. EFFECT OF COCAINE ON THE UTILIZATION OF CALCIUM IN ACETYLCHOLINE-INDUCED CONTRACTION OF ISOLATED VAS DEFERENS OF GUINEA PIG
• STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. V. CALCIUM AND CAFFEINE CONTRACTION OF SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM TAENIA COLI OF GUINEA PIG
• Norepinephrine : and K^+ : Induced Mn^ : Dependent Contractions and the Dynamics of Intracellular Mn^ Changes in Dispersed Smooth Muscle Cells From Ca^ : Depleted Mn^ : Loaded Vas Deferens of the Guinea Pig
• Effects of the Functional Elimination of Sarcoplasmic Reticulum on the Manganese-Dependent Norepinephrine-Induced Contractions of the Guinea Pig Vas Deferens.
• Role of bicarbonate ion in the postjunctional action of cocaine in the smooth muscle of the rat vas deferens.
• THE EFFECT OF DERIVATIVES OF IPTD ON BLOOD SUGAR AND GLUCAGON CONTENT OF PANCREAS
• Effects of various drugs on bladder function in conscious rats.
• Effects of various drugs on bladder function in conscious restrained-denervated rats placed in a restraining cage and produced by transection of the hypogastric nerve.
• Stimulatory Effects of HSR-803 on Ileal Motor Activity.
• Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.
• Evidence that Stretch Receptors for Peristalsis are Located in the Mucosal Layer of the Guinea Pig Ileum.

もっと見る 閉じる

スポンサーリンク