Norepinephrine : and K^+ : Induced Mn^<2+> : Dependent Contractions and the Dynamics of Intracellular Mn^<2+> Changes in Dispersed Smooth Muscle Cells From Ca^<2+> : Depleted Mn^<2+> : Loaded Vas Deferens of the Guinea Pig
スポンサーリンク
概要
- 論文の詳細を見る
The cellular mechanisms of norepinephrine (NE)-induced Mn2+-dependent contractions were investigated in dispersed smooth muscle cells from guinea pig vas deferens by characterizing the effects of NE and K+ on cell length observed by videotape microscopy and on intracellular Mn2+ and Ca2+ concentration ([Mn2+]i and [Ca2+]i) observed by confocal microscopy. Both stimulants induced slow sustained contraction in Ca2+-depleted, Mn2+-accumulated cells (Mn2+-loaded cells), whereas they induced biphasic contractions in normal cells that were neither Ca2+-depleted nor Mn2+-loaded. In both conditions, the number of cells responding to NE as well as the magnitude of NE-induced contractions increased in a dose-dependent manner. Contractions induced by K+ in Mn2+-loaded strip preparations were markedly smaller than those induced by NE. Although individual K+-induced contractions in responsive Mn2+-loaded cells were as large as those induced by NE, a much smaller percentage of Mn2+-loaded cells was responsive to K+ than to NE. These results are consistent with the idea that the contractions of strip preparations may reflect the magnitude of the contractions of individual cells as well as the percentage of responsive cells in the preparations. Inconsistent with the contractions, the [Mn2+]i rise induced by K+ was larger than that induced by NE, and the percentage of cells responsive to K+ was larger than that responsive to NE. These results suggest that NE may increase the Mn2+ sensitivity of contractile elements.
- 社団法人 日本薬理学会の論文
- 1998-11-01
著者
-
Gomi Yasuo
Department Of Pharmacology Faculty Of Pharmaceutical Science Kanazawa University
-
Tsunobuchi-ushijima Hiromi
Department Of Pharmacology Faculty Of Pharmaceutical Science Kanazawa University
-
IKOMA Kaoru
Department of Pharmacology, Faculty of Pharmaceutical Science, Kanazawa University
-
Ikoma Kaoru
Department Of Pharmacology Faculty Of Pharmaceutical Science Kanazawa University
関連論文
- PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. VII. INHIBITORY EFFECT OF DIBENAMINE ON COCAINE-INDUCED SUPERSENSITIVITY OF ISOLATED VAS DEFERENS OF GUINEA PIG
- Effects of Mazindol on the Contractions in the Rat Vas Deferens
- Spontaneous Tone in Different Types of Longitudinal Muscle Preparations of Guinea Pig Ileum
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS.VIII.^Ca-EFFLUX FROM SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM TAENIA COLI OF GUINEA PIG
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. IV. ISOLATION AND ACETYLCHOLINE-CONTRACTION OF SINGLE SMOOTH MUSCLE CELLS FROM TAENIA COLI OF GUINEA-PIG
- EFFECTS OF 1-(2-CHLORO-4-HYDROXYPHENYL)-t-BUTYL-AMINOETHANOL (HOKU-81), A NEW BRONCHODILATOR, ON ISOLATED TRACHEA AND ATRIA OF GUINEA PIG
- PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. VIII. DISSOCIATION OF INDUCTION OF SUPERSENSITIVITY FROM PRESYNAPTIC ACTION OF COCAINE ON ISOLATED VAS DEFERENS OF GUINEA PIG
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. VII. RESPONSE TO AGONISTS AND THE CONTRACTION VELOCITY OF TAENIA COLI OF GUINEA PIG AND THE SINGLE SMOOTH MUSCLE CELLS
- PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. XII. INHIBITORY EFFECT OF RESERPINE ON TRIPELENNAMINE-, N, N-DIMETHYL-N', N'-DIBENZYLEHYLENEDIAMINE- AND N, N-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE-INDUCED SUPERSENSITIVITY OF ISOLATED VAS DEFERENS OF G
- PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. XI. INHIBITORY EFFECT OF DIBENAMINE ON TRIPELENNAMINE-N, N-DIMETHYL-N', N'-DIBENZYLETHYLENEDIAMINE- AND N, N'-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE-INDUCED SUPERSENSITIVITY OF ISOLATED VAS DEFERENS OF G
- PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. X.EFFECT OF TRIPELENNAMINE, N, N-DIMETHYL-N', N'-DIBENZYLETHYLENEDIAMINE AND N, N-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE ON THE UTILIZATION OF CALCIUM IN ACETYLCHOLINE-INDUCED CONTRACTION OF ISOLATED VAS
- PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. IX. NONSPECIFIC SUPERSENSITIVITY OF VAS DEFERENS OF GUINEA PIG INDUCED BY TRIPELENNAMINE, N, N-DIMETHYL-N', N'-DIBENZYLETHYLENEDIAMINE AND N, N-DIBENZYL-N', N'-DIMETHYL-1,2-PROPANEDIAMINE
- Propranolol Blocks Cocaine-Induced Potentiation of the Contraction in the Smooth Muscle of the Rat Vas Deferens
- The Uptake of Manganese Induced by Agonists in the Isolated Vas Deferens of the Guinea Pig
- Effects of Ryanodine and Nifedipine on Contractions Induced by Norepinephrine, Acetylcholine, and Potassium in the Isolated Vas Deferens of the Guinea Pig
- Tachyphylaxis to the Inhibitory Effect of Manganese on Potassium-Induced Contractions in the Isolated Vas Deferens of the Guinea Pig
- PHARMACOLOGICAL STUDIES ON SUPERSENSITIZATION. VI. EFFECT OF COCAINE ON THE UTILIZATION OF CALCIUM IN ACETYLCHOLINE-INDUCED CONTRACTION OF ISOLATED VAS DEFERENS OF GUINEA PIG
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. V. CALCIUM AND CAFFEINE CONTRACTION OF SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM TAENIA COLI OF GUINEA PIG
- Norepinephrine : and K^+ : Induced Mn^ : Dependent Contractions and the Dynamics of Intracellular Mn^ Changes in Dispersed Smooth Muscle Cells From Ca^ : Depleted Mn^ : Loaded Vas Deferens of the Guinea Pig
- Effects of the Functional Elimination of Sarcoplasmic Reticulum on the Manganese-Dependent Norepinephrine-Induced Contractions of the Guinea Pig Vas Deferens.
- Role of bicarbonate ion in the postjunctional action of cocaine in the smooth muscle of the rat vas deferens.
- THE EFFECT OF DERIVATIVES OF IPTD ON BLOOD SUGAR AND GLUCAGON CONTENT OF PANCREAS
- Effects of various drugs on bladder function in conscious rats.
- Effects of various drugs on bladder function in conscious restrained-denervated rats placed in a restraining cage and produced by transection of the hypogastric nerve.
- Stimulatory Effects of HSR-803 on Ileal Motor Activity.
- Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.
- Evidence that Stretch Receptors for Peristalsis are Located in the Mucosal Layer of the Guinea Pig Ileum.