DISPOSITION OF OXPRENOLOL IN SPONTANEOUSLY HYPERTENSIVE RATS AS DETERMINED BY AN ENZYME IMMUNOASSAY

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Specific antisera against oxprenolol, a beta adrenoceptor blocking agent, were produced in rabbits immunized with oxprenolol hemiglutarate-bovine serum albumin conjugate. The antiserum, beta galactosidase labelled oxprenolol and 4-methylumbelliferyl-β-D-galactoside as substrate were used to develop an enzyme immunoassay for oxprenolol. The assay can reliably detect as little as one ng of oxprenolol directly from plasma. The plasma concentrations of oxprenolol in spontaneously hypertensive rats were determined by an enzyme immunoassay after a single and multiple oral doses for 14 consecutive days. Oxprenolol was rapidly absorbed from the gastrointestinal tract and was quickly eliminated from plasma. Plasma concentrations-time data conformed to equations describing one-or two-compartment open kinetic model. No significant differences in pharmacokinetic parameters were observed between after a single dose and multiple doses.
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