Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography
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概要
- 論文の詳細を見る
A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5'-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5,1.0,and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.
- 社団法人日本薬学会の論文
- 1983-05-25
著者
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松島 美一
Kyoritsu College of Pharmacy
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松島 美一
共立薬大
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菅田 節朗
Kyoritsu College of Pharmacy
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加留部 善晴
福岡大(薬)
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原 泰寛
Kyushu Cancer Center Research Institute
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加留部 喜晴
Kyushu Cancer Center Research Institute
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河野 彬
国立九州がんセンター
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田中 睦子
国立九州癌センター
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