Carboxypeptidases from the Exocarp of Mandarin Orange (Citrus unshiu MARC.). II. Chemical and Enzymatic Properties of Carboxypeptidases C_<Ua> and C_<Ub>
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概要
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Carboxypeptidases C_<Ua> and C_<Ub> were both shown to be glycoproteins by amino acid and carbohydrate analyses. The numbers of amino acid, hexose, and hexosamine residues were 811,26 (as galactose), and 6 (as glucosamine), respectively, for carboxypeptidase C_<Ua> and 868,42,and 8,respectively, for carboxypeptidase C_<Ub>. Both enzymes were almost completely inhibited by sodium dodecylsulfate and cetyltrimethyl ammonium bromide, but not by Brij-35,Tween 80,or Triton X-100. The enzymes were also inactivated by glycine ethyl ester in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, p-bromophenacyl bromide, phenylglyoxal, AgNO_3,CH_3HgCl, urea, and guanidine. When the enzymes were incubated with ^<203>Hg-labeled p-chloromercuribenzoic acid ([^<203>Hg] PCMB), approximately 0.2 mol of [^<203>Hg] PCMB was incorporated into them at pH 5.5 with no change in the enzymatic activity. After the denaturation of the enzymes with urea and guanidine, the incorporation of [^<203>Hg] PCMB increased to 2-3 mol per mol of enzyme. The values of K_m, V_<max>, and k_p for the hydrolysis of Z-Glu-Phe were 7.7×10^<-2>M, 222 μmol/l・min・mg, and 2.4×10^4 min^<-1>, respectively, for carboxypeptidase C_<Ua> and 7.1×10^<-2>M, 125 μmol/l・min・mg, and 1.6×10^4 min^<-1>, respectively, for carboxypeptidase C_<Ub>. Diisopropylfluorophosphate, phenylmethanesulfonyl fluoride and HgCl_2 inactivated the enzymes with decreases in the values of V_<max> and k_p but with no change in that of K_m, indicating that they are noncompetitive inhibitors of both enzymes. 3-Phenylpropionic acid, which inhibited the enzymes reversibly, increased the value of K_m without changing those of V_<max> and k_p and thus is a competitive inhibitor.
- 公益社団法人日本薬学会の論文
- 1980-12-25
著者
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庄司 省三
熊本大学 大学院医学薬学研究部薬学生化学分野
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久保田 幸穂
熊本大学薬学部
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船越 崇行
Faculty of Pharmaceutical Sciences, Kumamoto University
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庄司 省三
Faculty of Pharmaceutical Sciences, Kumamoto University
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植木 寛
Faculty Of Pharmaceutical Sciences Kumamoto University
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久保田 幸穂
Faculty Of Pharmaceutical Sciences University Of Tokyo
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森山 峰博
Faculty Of Pharmaceutical Sciences Kumamoto University
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