癌悪液質に関する生化学的研究(第2報) エールリッヒ腹水癌細胞およびマウス組織からの血清アルブミン低下物質の抽出

概要

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Hypoalbuminemic substances were partially purified from Ehrlich ascites carcinoma cells and from tissues of normal and tumor-bearing mice. The homogenate of tumor cells was extracted with saline, and the extract (F-1) obtained was fractionated by the addition of the same volume of a saturated solution of ammonium sulfate. After removal of the precipitate, the supernatant (F-2) was adjusted to pH 4.7 and fractionated again at 0.66 saturation of ammonium saulfate. The supernatant (F-3) thereby obtained showed a hypoalbuminemic activity. The decreases in total protein and albumin in the blood of mice, elicited by the intraperitoneal injection of 25-75 mg/kg of F-3,were statistica11y significant. The most pronounced decrease in total protein and albumin was produced 3 and 24 hr, respectively, after the injection of F-3. Qualitative ana1yses revealed the presence of protein, carbohydrate, lipid, and nuc1eic acid in F-3. The corresponding fractions obtained from liver and kidney of normal and tumor-bearing mice also showed hypoalbuminemic activity.
1974-01-25