Some Properties of Bovine Thymic Hypocalcemic Factor TP1

概要

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One of two hypocalcemic substances from the bovine thymus (TP1) was investigated. As a material for purification, it was confirmed by chromatography on diethylaminoethylcellulose that the fraction precipitated at 0-15% ammonium sulfate concentration was better than the 0-20% precipitate. Molecular weight of TP1 was 66000 by sedimentation equilibrium and this result agreed with that obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (68000) and suggested that TP1 was not composed of subunit. The circular dichroism spectrum of TP1 showed that the contents of α-helix, β-structure, and random form were 7,39 and 54%, respectively. TP1 contained 2.07 mol of the sulfhydryl residues by the mercury orange method. By the treatment at 56°for 30 min, hypocalcemic activity disappeared and the activity also disappeared by the treatment at 37°for 1 hr in acidic (pH 1.4) or alkaline (pH 12.6) solutions.
社団法人日本薬学会の論文
1977-12-25