Protection of Bovine Seryl-Transfer Ribonucleic Acid (Seryl-tRNA)Synthetase from Chemical Modification by Its Substrates, and Some Kinetic Parameters

概要

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Amino acid residues contained in the recognition sites of seryl-transfer ribonucleic acid (tRNA) synthetase (SerRS) were studied by chemical modification. Ser residues were modified with phenylmethanesulfolyl fluoride, and appeared to be unnecessary for the recognition. However, the modification of Arg residues with phenylglyoxal, His residues with diethylpyrocarbonate and sulfhydryl groups with 5,5'-dithiobis(2-nitrobenzoic acid), N-ethylmaleimede, iodoacetic acid or iodoacetamide showed that these residues were essential for the tRNA recognition by SerRS.Protection experiments with substrates from inactivation of Ser-tRNA formation by modification suggested that Arg residues interact with the γ-phosphate of adenosine triphosphate and tRNA.Modification of sulfhydryl groups showed that the groups interact with the hydroxyl groups of ribose of the CCA-end on tRNA. Furthermore, in order to understand the recognition mechanism between SerRS and tRNA^<Ser>, some kinetic parameters such as the K_m and V_<max> values of yeast tRNA^<Ser> and E. coli tRNA^<Ser> for bovine SerRS were compared with the values of bovine tRNA^<Ser>.
公益社団法人日本薬学会の論文
1988-10-25