Effects on Antibody-Producing Cells and Peripheral Bloody Lymphocyte Subpopulations (T- and B-cells) of P-MSY, a Substance with Antitumor Activity from Bovine Parotid Gland

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The effects of an antitumor protein P-MSY on the numbers of IgM producing cells and IgG producing cells were investigated. Groups of ICR-SLC newborn mice were injected intraperitoneally with P-MSY in doses of 1 to 10 μg/animal and their immune response was assessed at 21 days of age by measuring the antibody production in response to ovine erythrocytes according to the method of Jerne et al. The group of mice administered P-MSY at a dose of 1 μg/animal showed significant increases of the numbers of both direct PFC (IgM-producing cells) and indirect PFC (IgG-producing cells). The number of direct PFC of the test group on day 4 was 962.0 ± 153.8/10^6 cells, compared with 453.0±77.16/10^6 cells of the saline-treated control group. The number of indirect PFC of the test group on day 6 was 479.6±70.52/10^6 cells compared with 192.8±32.14/10^6 cells of the control group. Experiments were conducted to assess the effect of P-MSY on the peripheral blood lymphocyte subpopulations (T- and B-cells) in groups of newborn guinea pigs by identification and counting of T- and B-cells according to the microplate assay technique of Wilson et al. Guinea pigs dosed i.p. at the neonatal stage with 10 or 20 μg of P-MSY displayed a significant elevation of the T-cell percentage in peripheral blood with a significant relative decrease in the B-cell subpopulation (p≤0.05).
公益社団法人日本薬学会の論文
1981-11-25