Immune Competence-Promoting Action of Bovine Parotid Hypocalcemic Factor obtained via Extraction with Glacial Acetic Acid

概要

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The hypocalcemic substance P-MSY (M.W.66000) and a few partial purified products obtained through the glacial acetic acid extraction of bovine parotid extract were examined for immune competence. Lymphocytes/polymorphs ratio increasing action was examined using the littermates of Swiss-Webster strain mice, and plaque-forming cells increasing action and rosette-forming cells increasing action were done using those of ICR strain mice. By the dosage of 0.1 μg/mouse, the sample P-MSY produced L/P ratio of 2.72±0.12,and the effect was significant at 1% level of significance against the control group. PFC and RFC activities were significant at below 5% level of significance against the respective control group in a dose of 2.5 μg/mouse (PFC/10^6 cells : 53.57±14.57,RFC/10^6 cells : 13854±2156). From these results, L/P activity of P-MSY was increased by about 2000 times that of acetone-dried powder PAI, starting materials, and the increases in PFC and RFC activities were both about 80 times. Through the assay of P-MSY and partial purified products, a tendency to increase in L/P, PFC, and RFC activities was found in proportion of hypocalcemic activity. The regression of L/P and PFC activities against log doses were examined, and it was concluded that each response revolved to a line over a moderate range of doses. Meanwhile, P-MSY was compared with the series of other products from thymus.
公益社団法人日本薬学会の論文
1978-10-25