Sequence Determination of Amino- and Carboxyl-Terminal Residues of Hypocalcemic Profein TP_1 extracted from Bovine Thymus Gland
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概要
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End group amino acid sequence was determined on a hypocalcemic protein TP_1 purified from bovine thymus gland. Amino-terminal amino acid sequence was deduced to be His-Asx-Ala-Glx- by the dansyl-Edman degradation method. Carboxyl-terminal amino acid sequence was analyzed by digestion with carboxypeptidase A and Y, and it was deduced to be-Thr-Ala-Lys. Furthermore, the carboxyl-terminal amino acid was confirmed to be lysine alone by the hydrazinolysis method. According to these results and from previous data, it became apparent that TP_1 did not bear the subunit structure, and it was clearly different from other hypocalcemic proteins obtained from bovine thymus and parotid gland.
- 社団法人日本薬学会の論文
- 1978-09-25
著者
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田中 基裕
Faculty Of Pharmaceutical Science Nagoya City University
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水谷 彰
Faculty of Pharmaceutical Science, Nagoya City University
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水谷 彰
Faculty Of Pharmaceutical Sciences Nagoya City University:(present Address) The 2nd Division Daiyuka
関連論文
- Sequence Determination of Amino- and Carboxyl-Terminal Residues of Hypocalcemic Profein TP_1 extracted from Bovine Thymus Gland
- Some Properties of Bovine Thymic Hypocalcemic Factor TP1
- Effect of Bovine Thymic Hypocalcemic Factor on Increasing Antibody-producing Cells in Mice
- Purification and Characterization of Hypocalcemic Protein from Porcine Parotid Gland
- An Active Core in a Limited Chymotryptic Digest of Bovine Parotid Hypocalcemic Substance
- Effect of Hypocalcemic Protein P-MSY obtained from Bovine Parotid Gland on the Cyclic Adenosine-5'-monophosphate and Cyclic Guanosine-5'-monophosphate Contents of Murine Thymus and Spleen Cells
- Antitumor Activity of P-MSY, a Protein from Bovine Parotid Glands, and Some Observations relating to the Activity
- Effects on Antibody-Producing Cells and Peripheral Bloody Lymphocyte Subpopulations (T- and B-cells) of P-MSY, a Substance with Antitumor Activity from Bovine Parotid Gland
- Purification and Characterization of Bovine Parotid Hypocalcemic Factor obtained via Extraction with Glacial Acetic Acid
- Antitumor Activity of Bovine Parotid Hypocalcemic Substance Obtained by Glacial Acetic Acid Extraction
- Immune Competence-Promoting Action of Bovine Parotid Hypocalcemic Factor obtained via Extraction with Glacial Acetic Acid
- Evidence for Non-mediation of Calcitonin in the Action of Hypocalcemic Protein from Bovine Parotid Gland
- Chemical Modification of the Bovine Parotid Hypocalcemic Protein
- Circular Dichroism of a Hypocalcemic Protein in Bovine Parotid Gland
- Effect of Parotid Gland Hypocalcemic Factor on Increasing Antibody-producing Cells in Mice
- Amino- and Carboxyl-terminal Sequences of a Hypocalcemic Protein Isolated from Bovine Parotid Gland
- Molecular Weight Determination and the Secondary Structure of the Hypocalcemic Protein purified from Bovine Parotid Gland
- Isolation of a Hypocalcemic Substance in Bovine Parotid Gland, and Some Properties of this Substance
- The Influence of Bovine Thymus Hypocalcemic Protein, TP1,on the Levels of Cyclic Nucleotides in Murine Thymus Lymphocytes
- Isolation of a Deoxyribonuclease from Bovine Parotid Gland