Effect of Some Aminoquinone Compounds on Nucleic Acid Synthesis catalyzed by E. coli DNA Polymerase I and RNA Polymerase

概要

論文の詳細を見る
The effect of DNA-interacting aminoquinones, 2-amino-1,4-naphthoquinone imine (ANQI), 2,5-diamino-1,4-naphthoquinone imine (DANQI) and 2-hydroxyamino-1,4-naphthoquinone (HANQ), which had been reported to cellular DNA synthesis in Ehrlich ascites carcinoma and E. coli, was studied on cell-free DNA and RNA syntheses catalyzed by E. coli DNA polymerase I and RNA polymerase, respectively. These aminoquinones inhibited both the polymerase reactions to a similar degree (30-56% at 1×10^<-3>M). The inhibition of DNA synthesis was in nearly the same extent as that observed in E. coli whole cells, but lower than that found in intact cells of Ehrlich ascites carcinoma. The inhibition of the DNA polymerase reaction by the aminoquinones was competitive with calf thymus DNA used as the template. Any of the aminoquinones received higher spectral change by the native DNA than by the heat-denatured, and also more inhibited the DNA synthesis directed by the double-stranded DNA than that by the single-stranded. These results suggest that the inhibition is due to the interaction between the DNA and the aminoquinones.
公益社団法人日本薬学会の論文
1977-11-25