ネコブセンチュウ(Meloidogyne incognita var. acrita)の生理的食塩水内産卵について

概要

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This study examined the applicability of the female adults of the root-knot nematode, Meloidogyne incognita var. acrita to biological experiments. The 3.3-70 atm. osmotic pressure of NaCl and sucrose solutions appeared to be identical that of the body fluid. In other solutions of lower or higher osmotic pressures, the body ruptured or shrank, respectively. It was found that the most suitable solution for the female to spawn was as follows: NaCl 0.80g, KCl 0.01g, CaCl_2 0.01g, NaHCO_3 0.02g dissolved in 100ml of H_2O. Dehydroacetic acid or its sodium salt was added to this solution (400 ppm) as a fungicide. In this culture medium the spawned eggs did not aggregate, but were dispursed and easily counted. Members of eggs spawned by individual female was markedly varied even among individuals with about the same body length and width. But the deviation in number was small in females which produced white egg masses; these females laid more eggs than those spawning yellow or brown egg masses. The optimal temperature of the culture medium for spawning was about 25℃. The lowest critical temperature was 10℃. At this temperature the females maintained their reproductive activity for 9 days. Applicability of this nematoda to biological experiments using their female adultswas discussed and several improvements in culturing technique were suggested.
社団法人日本動物学会の論文
1963-06-15