イモリ胚予定外胚葉の第一次反応能の消失

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Change in the primary competence of isolated ectoderm of newt embryos was studied. The ectodermal pieces were isolated from embryos at stage 12 (Okada and Ichikawa 1947), and prior to the application of inductor they were cultured in Holtfreter's solution at room temperature for several hours, up to 28 hours. The inductors were extracted from guinea pig thigh bone marrow or liver and precipitated by an addition of 2 volumes of cold ethanol. The archencephalon was induced by liver extract but the rate of the induction diminished progressively with the increase in the time of culture of the ectoderm. The decline of the induction rate became remarkable at 12 hours of culture, and after that the frequency of forebrain formation decreased, and unorganized neural tissues became predominant. After 24 hours, the explants became atypical epidermis, except for unorganized small neural tissue evoked in a few cases. Mesodermal tissues were induced by bone marrow and the change in the induction rate was similar to that in the rate of neural induction by liver. In this case, however, the change started somewhat earlier, at about 7 hours of culture. At an earlier time of culture, the formation of the dorsal mesodermal tissues like notochord and muscle predominated over the formation of the ventral mesodermal tissue like mesenchyme. This relation was reversed as the induction rate decreased. After 24 hours, no mesodermal tissues were evoked in explants.
社団法人日本動物学会の論文
1975-06-25

イモリ胚予定外胚葉の第一次反応能の消失

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