ウニ精子の鞭毛を構成するフィラメントのヌクレオチドについて

概要

論文の詳細を見る
In a previous experiment (Mohri, 1964), it was noted that a KCl-extract of sea-urchin sperm flagella contain some nucleotides, absorption curve of which has a maximum at 258 mμ and a shoulder around 280 mμ, thus resembling guanosine nucleotides. The KCl-extract comprises flagellar ATPase and also some proteins originated from the filaments (or microtubules) making up the so-called "9+2" structure of flagella. In the present experiment, therefore, "9+2" filaments were isolated and their nucleotides were examined. A TCA-extract of the filaments was put on a Dowex 1, x80 (200-400 mesh) column and effluents were analyzed chemically and spectro photometrically. Three peaks were obtained and absorption spectrum of each peak exhibited characteristics of guanosine nucleotides. Chemical analyses showed that they correspond to GMP, GDP and GTP, respectively. The results is a sharp contrast to the fact that most of nucleotides found in the TCA-extract of whole sperm cells are adenosine nucleotides, especially ATP. The guanosine nucleotides seem to be firmly bound to the filament proteins, possibly one nucleotide to one monomeric unit of the filament, while adenosine nucleotides diffuse in a free atate in the other part of flagella.
社団法人日本動物学会の論文
1967-08-15