The feeding regulation in the hypothalamic neurons
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概要
- 論文の詳細を見る
The hypothalamus has been recognized as a central region of feeding regulation. It has been shown by some physiological evidences that the ventromedial nucleus (VMH) is a satiety center and the lateral hypothalamus (LH) is a feeding center. Recently it was demonstrated that the arcuate nucleus (ARC) acts as a feeding controlling center, because recent studies have revealed that proopiomelanocortin (POMC) - containing neurons in the ARC are thought to function as feeding inhibitors, while neuropeptide Y (NPY) - containing neurons are thought to function in feeding stimulation. More recently some new feeding-regulating factors were discovered. Leptin (LP) is the hormone that is secreted by adipocytes, transported into the hypothalamus and exerts feeding inhibition. Orexin (OX) is the feeding stimulating peptide that is expressed exclusively around the LH region and has its neural projections throughout the brain. Ghrelin (GL) is also considered as peptide involved in stimulating food intake and is synthesized in the ARC as well as in stomach. The present study was designed to clarify the neuron network of these factors and these receptors for the feeding regulation in the hypothalamus. The distribution of these new candidates in the rat brain were determined by immunohistochemistry. Then the interactions among these expressing neurons were examined by double immunostaining methods at the light and electron microscopic level. In addition, we studied signal transduction mechanism in isolated feeding-regulating neurons by UP, OX, and GL. Strong leptin receptor (LP-R) immunoreactivity was detected in the VMH, LH, and ARC. In the double immunofluorescence method, it was revealed that LP-R was expressed in the many OX-, NPY-, and POMC- containing neurons in the hypothalamus. At the ultrastructural level, it was demonstrated that OXergic axon terminals in the ARC, make synapses on NPY and POMC neurons, and that there are reciprocal synaptic relationships between the OX- and GL- containing neurons. Cytosolic Ca^<2+> concentration ( [Ca^<2+>]_i) in single neurons isolated from the ARC was measured with dual wavelength fura-2 fluorescence imaging. LP decreased and OX increased [Ca^<2+>]_i in NPY neurons. Inversely, LP increased and OX decreased [Ca^<2+>]_i in POMC neurons. GL increased [Ca^<2+> ]_i in NPY neurons. In the present study, it was elucidated that there were very complex networks among many neurons expressing various morecules on the feeding regulation in the LH and ARC. The feeding regulation with satiety neurons in the VMH is interesting study in future.
- 日本組織細胞化学会の論文
著者
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Shioda Seiji
Department Of Anatomy Showa University School Of Medicine
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Shioda S
Department Of Anatomy Showa University School Of Medicine
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SHIODA Seiji
Showa University School of Medicine
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Shioda S
Showa University School Of Medicine
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Guan Jian
Department of Anatomy, Showa University School of Medicine
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Funahashi Hisayuki
Department of Anatomy, Showa University School of Medicine
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GUAN Jian-Lian
Department of Anatomy, Showa University School of Medicine and the Core Research for Evolutional Sci
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YADA Toshihiko
Department of Physiology, Jichi Medical School
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Kono Daisuke
Department Of Physiology Jichi Medical School
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Shioda Seiji
Department Of Anatomy School Of Medicine Showa University
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Funahashi Hisayuki
Department Of Anatomy Showa University School Of Medicine
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Funahashi Hisayuki
Department Of Anatomy School Of Medicine Showa University
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Yada Toshihiko
Department Of Physiology Division Of Integrative Physiology Jichi Medical School School Of Medicine
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Yada Toshihiko
Department Of Physiology Jichi Medical School
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Guan J‐l
Department Of Anatomy Showa University School Of Medicine
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Kono Daisuke
Department Of Micro Engineering Graduate School Of Engineering Kyoto University
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Guan Jian-Lian
Department of Anatomy, Showa University School of Medicine
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