IMMUNOHISTOCHEMISTRY OF SEROTONIN (5-HT) IN THE HUMAN GUT ENDOCRINE CELLS : AN APPLICATION OF ULTRASTRUCTURAL IMMUNOHISTOCHEMISTRY
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概要
- 論文の詳細を見る
The ultrastructural localization of serotonin (5-HT)-like immunoreactivity was investigated in human gut endocrine cells by the block-staining method using horseradish peroxidase (HRP)-labeled anti-5-HT rabbit (Fab')_2. The reactive products against 5-HT were satisfactorily demonstrated on the membrane-bound and/or non-membrane-bound ribosomal granules as well as on the basally accumulated biconcave granules as seen in typical entero-chromaffin (EC) cells. The cross-reactivity of the anti-5-HT antisera to various tryptophan related compounds and the other monoamines was examined by the ^<125>I-labeled protein A (IPA) assay system. The anti-5-HT antisera were completely neutralized by serotonin-bovine serum albumin (BSA) and N-acetylserotonin-BSA conjugates and incompletely by free serotonin creatininesulfate. No inhibition of the antibody binding activity was observed when the antisera were preincubated with tryptophan and its related compounds or other monoamines. In conclusion, our results confirmed the ultrastructural localization of 5-HT-like immunoreactivity in EC cells and the availability of the block-staining method using HRP-labeled anti-5-HT (Fab')_2 for ultrastructural immunohistochemistry.
- 日本組織細胞化学会の論文
著者
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Kawaoi Akira
Department of Pathology, Yamanashi Medical University
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Kawaoi Akira
Department Of Pathology Yamanashi Medical School
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Sako Hiroko
1st Department Of Pathology Nihon University School Of Medicine
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Nemoto Norimichi
Department Of Iind Pathology Nihon University School Of Medicine
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Kawaoi Akira
Department Of Pathology Nihon University School Of Medicine
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Shikata Toshio
1st Department Of Pathology Nihon University School Of Medicine
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Shikata Toshio
First Department Of Pathology Nihon University School Of Medicine
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OKANO Tadao
Department of Ist Pathology, Nihon University School of Medicine
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NEMOTO NORIMICHI
First Department of Pathology, Nihon University School of Medicine
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OKANO TADAO
First Department of Pathology, Nihon University School of Medicine
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USHIYAMA HISASHI
First Department of Pathology, Nihon University School of Medicine
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SATOH HIDEKO
First Department of Pathology, Nihon University School of Medicine
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Shikata Toshio
Department Of Ist Pathology Nihon University School Of Medicine
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Nemoto Norimichi
Department Of Ist Pathology Nihon University School Of Medicine
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Okano Tadao
1st Department Of Pathology Nihon University School Of Medicine
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Okano Tadao
First Department Of Pathology Nihon University School Of Medicine
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Ushiyama Hisashi
1st Department Of Pathology Nihon University School Of Medicine
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Sato Hideko
1st Department Of Pathology Nihon University School Of Medicine
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SHIKATA Toshio
Department of Ist Pathology, Nihon University School of Medicine
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