EVALUATION OF MICROWAVE IRRADIATION IN IMMUNOHISTOCHEMICAL REACTIONS (<Symposium>Microwave Fixation in Histochemistry)
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概要
- 論文の詳細を見る
The usefulness of the specimens prepared following fixation by microwave (MW) irradiation in immunohistochemical reactions was evaluated using various antibodies. The results showed that in the case of the content of secretory granules, the antigenicities were found to be preserved well along with improved structural preservation by MW irradiation, and specimens with excellent localization were obtained. The antigenicity of basement membrane components could always be detected when tissues were fixed by MW irradiation with water soluble carbodiimide (WSC) added as a fixative. For plasma membrane antigens and cytoskeleton antigens, essentially the same results were obtained by fixation with MW irradiation and conventional fixation by immersion. There thus appears the possibility that the use of new fixatives may render MW irradiation more useful for immunohistochemical reactions.
- 日本組織細胞化学会の論文
著者
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Yamashina Shohei
Department Of Anatomy And Cell Biology Kitasato University School Of Medicine
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Katsumata Osamu
Dept. of Anatomy, kitasato University, School of Medicine
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Katsumata O
Kitasato Univ. Kanagawa Jpn
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Katsumata Osamu
Department Of Anatomy And Cell Biology Kitasato University School Of Medicine
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Sekine Reiko
Department Of Anatomy Kitasato University School Of Medicine
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Yamashina Shohei
Departmant Of Anatomy And Cell Biology Tokyo Medical And Dental University
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