HISTOCHEMICAL DETECTION TECHNIQUE OF GLUCOSE OXIDASE
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概要
- 論文の詳細を見る
Glucose oxidase in mold and rat kidney was demonstrated histochemically by means of a combined utilization of ferricyanide and lead ions. Brownish deposits of sulfurated lead ferrocyanide were found on the cell walls of the mold and in glomeruli and intertubular spaces of the kidney. It is presumed that the reducing activity of hydrogen peroxide generated during the enzymatic oxidation of glucose by glucose oxidase changes the ferricyanide to the ferrocyanide ion, which, reacting with the lead ion, makes whitish precipitates in the tissue. Since the results indicate that hydrogen peroxide can be captured histochemically, the technique may be applicable to the histochemical detection of other kinds of oxidase if suitable substrate substitution are made. Furthermore, it may be expanded to electron microscopic histochemistry.
- 日本組織細胞化学会の論文
著者
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Yamashina Shohei
Departmant Of Anatomy And Cell Biology Tokyo Medical And Dental University
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Yamashina Shohei
Department Of Anatomy And Cell Biology School Of Medicine Tokyo Medical And Dental University
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