THE USE OF GRIFFONIA SIMPLICIFOLIA AGGLUTININ-IB_4 IN STAINING THE DEVELOPING RAT FUNDIC GLAND

概要

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The development of Wistar rat fundic glands was studied using Griffonia simplicifolia agglutinin-IB_4 (GSA-IB_4) at the light microscopic and electron microscopic levels. In adult rats, GSA-IB_4 specifically labeled the intracellular canaliculi and theapical surface on the fundic gland parietal cells, whereas the other sites of these cells were very weakly or rarely labeled. Ontogenic studies revealed that at 18 days of gestation, GSA-IB_4 positive reaction was found along the whole cell surface of the cells on the fundic gland and immature intracellular canaliculi of the primitive parietal cells. At 1 day after birth, the positive reaction was restricted to the parietal cells. GSA-IB_4 specifically labeled the intracellular canaliculi and the apical cell surface of the parietal cells, but the labeling of the basolateral cell membrane of parietal cells decreased. At the point of weaning, 3 weeks after birth, GSA-IB_4 strongly labeled the intracellular canaliculi whereas the basolateral membrane was weakly labeled. This staining pattern did not change basically until adult stage. From these results, it is concluded that the functional maturation of the parietal cells may occur at weaning in spite of their early appearance of morphological characters.
日本組織細胞化学会の論文