ELECTRON MICROSCOPIC RADIOAUTOGRAPHY USING A COMBINATION OF PHENIDON DEVELOPER AND DOMESTIC EMULSION

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概要

• 論文の詳細を見る

• A routine procedure for electron microscopic radioautography in combination with phenidon developer and the domestic emulsion, Sakura NR-H2, was developed. Good stable radioautograms have not been obtained by simply developing the emulsion coated grids with phenidon developer at any temperature, but the development of these grids at 18℃ for 1 min after 30-45 sec gold latensification in freshly prepared gold thiocyanate gives satisfactory results. Some electron microscopic radioautograms developed by this method are presented. The addition of the anionic surfactant, dioctyl sodium sulphosuccinate, to the emulsion was found to be very effective not only for preventing the burst of emulsion but also in obtaining a uniform spread of the emulsion for the wire loop method. Radioautograms obtained with this method are suitable for quantitative electron microscopic radioautographic studies. Details of this method are introduced.

• 日本組織細胞化学会の論文

著者

• Murata Fusayoshi

  Department of Structural Cell Biology, Kagoshima University Graduate School of Medical and Dental Sc

• Murata Fusayoshi

  Department Of Anatomy Faculty Of Medicine Kagoshima University

• Murata Fusayoshi

  Kagoshima University School Of Medicine

• YOSHIDA KEIZO

  Shinshu University School of Medicine

• OHNO SHINICHI

  Shinshu University School of Medicine

• NAGATA TETSUJI

  Shinshu University School of Medicine

• OHNO Shinichi

  1st Department of Anatomy, Shinshu University School of Medicin

• Murata Fusayoshi

  Department Of Anatomy Shinshu University School Of Medicine

• Ohno Shinichi

  1st Department Of Anatomy Shinshu University School Of Medicin

• Nagata Tetsuji

  Department Of Anatomy Shinshu University School Of Medicine

• Nagata Tetsuji

  Shinshu Univ. School Of Medicine Nagano Jpn

• Nagata Tetsuji

  1st Department Of Anatomy Shinshu University School Of Medicin

• Ohno Shinichi

  Department Of Anatomy Shinshu University School Of Medicine

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