ELECTRON MICROSCOPIC RADIOAUTOGRAPHY USING A COMBINATION OF PHENIDON DEVELOPER AND DOMESTIC EMULSION
スポンサーリンク
概要
- 論文の詳細を見る
A routine procedure for electron microscopic radioautography in combination with phenidon developer and the domestic emulsion, Sakura NR-H2, was developed. Good stable radioautograms have not been obtained by simply developing the emulsion coated grids with phenidon developer at any temperature, but the development of these grids at 18℃ for 1 min after 30-45 sec gold latensification in freshly prepared gold thiocyanate gives satisfactory results. Some electron microscopic radioautograms developed by this method are presented. The addition of the anionic surfactant, dioctyl sodium sulphosuccinate, to the emulsion was found to be very effective not only for preventing the burst of emulsion but also in obtaining a uniform spread of the emulsion for the wire loop method. Radioautograms obtained with this method are suitable for quantitative electron microscopic radioautographic studies. Details of this method are introduced.
- 日本組織細胞化学会の論文
著者
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Murata Fusayoshi
Department of Structural Cell Biology, Kagoshima University Graduate School of Medical and Dental Sc
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Murata Fusayoshi
Department Of Anatomy Faculty Of Medicine Kagoshima University
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Murata Fusayoshi
Kagoshima University School Of Medicine
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YOSHIDA KEIZO
Shinshu University School of Medicine
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OHNO SHINICHI
Shinshu University School of Medicine
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NAGATA TETSUJI
Shinshu University School of Medicine
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OHNO Shinichi
1st Department of Anatomy, Shinshu University School of Medicin
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Murata Fusayoshi
Department Of Anatomy Shinshu University School Of Medicine
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Ohno Shinichi
1st Department Of Anatomy Shinshu University School Of Medicin
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Nagata Tetsuji
Department Of Anatomy Shinshu University School Of Medicine
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Nagata Tetsuji
Shinshu Univ. School Of Medicine Nagano Jpn
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Nagata Tetsuji
1st Department Of Anatomy Shinshu University School Of Medicin
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Ohno Shinichi
Department Of Anatomy Shinshu University School Of Medicine
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