PEROXISOMAL PROLIFERATION IN RAT KIDNEY INDUCED WITH DEHP I. NUMERICAL CHANGE BY LIGHT MICROSCOPIC MORPHOMETRY
スポンサーリンク
概要
- 論文の詳細を見る
Effects of DEHP (di-2-ethylhexyl phthalate) were studied on renal tubular epithelial cells of rats. The 1%, 2% and 4% (w/w) DEHP administered and control rats were sacrificed at 1, 2 and 4 weeks. The tissues were incubated in alkaline DAB medium and embedded in Epon. The 0.5 μm thick sections were observed by light microscopy with an ocular micrometer. The number of peroxisomes was determined for the transected area per 25 μm^2 in 40 different tubular cytoplasms. The differences in the number of peroxisomes between the experimental and control groups were evaluated from the viewpoint of various responses depending on the duration of administration, dose of DEHP, parts of the nephron and sex. Peroxisomal proliferation in DEHP-treated rats occured at low levels of dosage in a short time. The differences in increase between straight parts and convoluted parts of proximal tubules became the most obvious over a long time. The difference depending on sex was not so apparent. The response of proximal tubular epithelial cells to DEHP is not similar to that induced in liver cells by the same drug. Peroxisomes in renal tubular epithelial cells may play a role in lipid metabolism to a lesser extent than those in liver cells.
- 日本組織細胞化学会の論文
著者
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Murata Fusayoshi
Kagoshima Univ.
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Usuda Nobuteru
1st Department Of Anatomy Shinshu University School Of Medicin
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Usuda Nobuteru
Department Of Anatomy And Cell Biology Shinshu University School Of Medicine
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OHNO Shinichi
Department of Anatomy, Yamanashi Medical University School of Medicine
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FUJII Yasuhisa
Department of Urology, Tokyo Medical and Dental University, Graduate School
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Fujii Yasuhisa
Department Of Anatomy Shinshu University School Of Medicine
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NAGATA Tetsuji
Department of Anatomy and Cell Biology, Shinshu University School of Medicine
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Murata Fusayoshi
Kagoshima University School Of Medicine
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Murata Fusayoshi
Kagoshima Medical Technology College
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OHNO Shinichi
1st Department of Anatomy, Shinshu University School of Medicin
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Murata Fusayoshi
Department Of Anatomy Shinshu University School Of Medicine
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Ohno Shinichi
1st Department Of Anatomy Shinshu University School Of Medicin
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Nagata Tetsuji
Department Of Anatomy Shinshu University School Of Medicine
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Nagata Tetsuji
Departmenrts Of Anatomy And Cell Biology Shinsyu University School Of Medicine
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Usuda Nobuteru
Departmenrts Of Anatomy And Cell Biology Shinsyu University School Of Medicine
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Nagata Tetsuji
1st Department Of Anatomy Shinshu University School Of Medicin
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Ohno Shinichi
Department Of Anatomy And Molecular Histology Interdisciplinary Graduate School Of Medicine And Engi
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Ohno Shinichi
Department Of Anatomy Shinshu University School Of Medicine
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Fujii Yasuhisa
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898
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