Immunohistochemical Application of Cryotechniques to Native Morphology of Cells and Tissues(Yong Histochemist of the Year)
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概要
- 論文の詳細を見る
Common immunohistochemistry is a technique based on antigen-antibody reactions in thin sections. It is well known that the important steps of tissue preparation protocols are (i) fixation (chemical and physical methods, especially cryofixation), (ii) sectioning, and (iii) immunostaining. We have already examined how to combine these preparation steps for an improved immunohistochemistry of target cells and tissues so as to overcome some of the technical problems commonly encountered. In many experimental cases, various cryotechniques were useful to obtain both a strong immunoreactivity of the antigens and a high time-resolution of the morphology of living animal cells and tissues. When the ultimate aim is to obtain in vivo observation of the morphology and immunolocalization, the new concept of in vivo cryotechnique becomes indispensable in the field of cell biology.
- 日本組織細胞化学会の論文
著者
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OHNO Shinichi
Department of Anatomy, Yamanashi Medical University School of Medicine
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TERADA Nobuo
Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of
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Terada Nobuo
Department Of Anatomy Interdisciplinary Graduate School Of Medicine And Engineering University Of Ya
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Terada Nobuo
Department Of Anatomy And Molecular Histology Interdisciplinary Graduate School Of Medicine And Engi
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Ohno Shinichi
Department Of Anatomy And Molecular Histology Interdisciplinary Graduate School Of Medicine And Engi
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Ohno Shinichi
Department Of Anatomy Interdisciplinary Graduate School Of Medicine And Engineering University Of Ya
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