Homeostatic Mass Control in Gastric Non-Neoplastic Epithelia under Infection of Helicobacter pylori: An Immunohistochemical Analysis of Cell Growth, Stem Cells and Programmed Cell Death
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概要
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We evaluated homeostatic mass control in non-neoplastic gastric epithelia under Helicobacter pylori (HP) infection in the macroscopically normal-appearing mucosa resected from the stomach with gastric cancer, immunohistochemically analyzing the proliferation, kinetics of stem cells and programmed cell death occurring in them. Ki67 antigen-positive proliferating cells were found dominantly in the elongated neck portion, sparsely in the fundic areas and sporadically in the stroma with chronic infiltrates. CD117 could monitor the kinetics of gastric stem cells and showed its expression in two stages of gastric epithelial differentiation, namely, in transient cells from the gastric epithelial stem cells to the foveolar and glandular cells in the neck portion and in what are apparently progenitor cells from the gastric stem cells in the stroma among the infiltrates. Most of the nuclei were positive for ssDNA in the almost normal mucosa, suggesting DNA damage. Cleaved caspase-3-positive foveolar cells were noted under the surface, suggesting the suppression of apoptosis in the surface foveolar cells. Besides such apoptosis of the foveolar cells, in the severely inflamed mucosa apoptotic cells were found in the neck portion where most of the cells were Ki67 antigen-positive proliferating cells. Beclin-1 was recognized in the cytoplasm and in a few nuclei of the fundic glandular cells, suggesting their autophagic cell death and mutated beclin-1 in the nuclei. Taken together, the direct and indirect effects of HP infection on the gastric epithelial proliferation, differentiation and programmed cell death suggested the in-situ occurrence of gastric cancer under HP infection.
- 日本組織細胞化学会の論文
著者
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Aikou Takashi
Department Of 1st Surgery Faculty Of Medicine
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Murata Fusayoshi
Department Of Anatomy Faculty Of Medicine Kagoshima University
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Wang Jia
Department Of Microbiology Peking University Health Science Center
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Hasui Kazuhisa
Department Of Anatomy Kagoshima University Faculty Of Medicine
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Kato Kenji
Department Of Biology And Geosciences Faculty Of Science Shizuoka University
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Kawano Yoshifumi
Department of Child Health, Graduate School of Comprehensive Human Sciences, University of Tsukuba
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Kawano Yoshifumi
Department of Pediatrics, Field of Developmental Medicine, Course of Health Research, Kagoshima University Graduate School of Medical and Dental Sciences
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Murata Fusayoshi
Department of Structural Cell Biology (Previous Second Department of Anatomy), Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Sciences
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Hasui Kazuhisa
Department of Immunology (Previous Second Department of Anatomy), Field of Infection and Immunity, Course of Health Research, Kagoshima University Graduate School of Medical and Dental Sciences
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Wang Jia
Department of Immunology (Previous Second Department of Anatomy), Field of Infection and Immunity, Course of Health Research, Kagoshima University Graduate School of Medical and Dental Sciences
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