Estimation of "Dehalococcoides" Populations in Lake Sediment Contaminated with Low Levels of Polychlorinated Dioxins
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概要
- 論文の詳細を見る
Vertical profiles of polychlorinated dioxins and microbial biomass including "Dehalococcoides" populations in cores of sediment of Lake Suwa, Japan, were investigated. The core samples were analyzed in 3-cm intervals at 0-15cm and 50cm, where a sharp gradient of E_h from 5 to-110mV with depth occurred. The concentration of polychlorinated dioxins was relatively constant at 0-15cm, ranging from 7.6 to 8.3ng (7.0-9.2 pg-TEQ [toxic equivalent]) g^<-1> dry wt, but decreased sharply at 50cm. The total bacterial count was in the order of 10^8 to 10^9 g^<-1> dry wt, being highest at 3-6cm and decreasing in the deeper sediment. A similar vertical profile was found for respiratory quinones with larger amounts of ubiquinones than menaquinones at 0-15cm. Quantitative real-time PCR with a specific primer set showed that "Dehalococcoides" and its phylogenetic relatives occurred in the order of 10^4 g^<-1> (dry wt) at 0-12cm but were absent at 50cm. The amplified clones showed 91-100% similarity (mostly <94%) in sequence to a well-known dioxin-dechlorinating organism, "Dehalococcoides" sp. strain CBDB1. These results suggested that the surface sediment up to a depth of 12cm provided favorable conditions for the growth and activity of both aerobic and anaerobic microorganisms including "Dehalococcoides". It is likely that a wide variety of "Dehalococcoides" and phylogenetic relatives thereof are omnipresent even in sediment with low levels of dioxins and play the primary role in dechlorinating organohalorides over a relatively wide range of E_h.
- 日本微生物生態学会の論文
著者
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HIRAISHI AKIRA
Department of Ecological Engineering, Toyohashi University of Technology
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KATO Kenji
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University
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Futamata Hiroyuki
Department of Ecological Engineering, Toyohashi University of Technology
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Hiraishi A
Toyohashi Univ. Technol. Aichi Jpn
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Hiraishi Akira
Department Of Ecological Engineering Toyohashi University Of Technology
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Hiraishi Akira
Tokyo Research Laboratory Konishi Co. Ltd. :(present Address)institute Of Applied Microbiology Unive
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MARUYAMA TOMOKO
Department of Geosciences, Faculty of Science, Shizuoka University
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Futamata Hiroyuki
Department Of Ecological Engineering Toyohashi University Of Technology:(present Office)department O
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Futamata Hiroyuki
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Futamata Hiroyuki
Department Of Material Science And Chemical Engineering Shizuoka University
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Futamata Hiroyuki
Department Of Ecological Engineering Toyohashi University Of Technology
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Kato Kenji
Graducate School Of Science And Technology Shizuoka University:graduate School Of Science Shizuoka U
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Maruyama Tomoko
Department Of Biology And Geosciences Faculty Of Science Shizuoka University
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Kato Kenji
Laboratory of Earth Microbiology, Department of Geosciences, Faculty of Science, Shizuoka University
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Hashizume Akiko
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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MIYAKODA HIDEKI
Sinto Environmental Measurement Centre, Shintokogyo Ltd.
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SAKAMAKI NORIKO
Department of Ecological Engineering, Toyohashi University of Technology
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Futamata H
Department Of Ecological Engineering Toyohashi University Of Technology
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Sakamaki Noriko
Department Of Ecological Engineering Toyohashi University Of Technology
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Miyakoda Hideki
Sinto Environmental Measurement Centre Shintokogyo Ltd.
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Hiraishi Akira
Department Of Biology Faculty Of Science Tokyo Metropolitan University
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Kato Kenji
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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Kato Kenji
Department Of Biology And Geosciences Faculty Of Science Shizuoka University
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