Biotransformation of Polychlorinated Dioxins and Microbial Community Dynamics in Sediment Microcosms at Different Contamination Levels
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概要
- 論文の詳細を見る
Semi-anaerobic microcosms containing different levels of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed by seeding with different mass ratios of lake sediment and dioxin-contaminated soil and incubating with organic medium for 1 year. In all microcosms, PCDD/Fs were reduced as a first-order reaction with similar removal rate coefficients, and only trace amounts of less chlorinated congeners were produced as the intermediate and end products. This apparent complete dechlorination of PCDD/Fs seemed to be due to a combination of reductive dechlorination of PCDD/Fs and oxidative degradation of the dechlorinated products. Total cell counting, 16S rRNA gene clone library analyses and quinone profiling showed that the microcosms contained relatively constant total populations with members of the phyla Bacteroidetes, Firmicutes and Proteo-bacteria (especially "Deltaproteobacteria") as the major constituents, independent of pollution levels. Quantitative real-time PCR with a specific primer set showed that the population density of "Dehalococcoides" and its phylogenetic relatives was highly correlated with the concentration of PCDD/Fs present. Some "Dehalococcoides" strains were isolated from the microcosms by repeated enrichment with chloroaromatics as the terminal electron acceptor. However, these isolates did not match with the major "Dehalococcoides"-related clones directly PCR-amplified. The results of this study suggest that PCDD/Fs in natural environments under given conditions are transformed with similar half-reduction rates independent of their concentrations, and a wide variety of "Dehalococcoides"-related bacteria play the primary role in this process.
- 日本微生物生態学会の論文
著者
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HIRAISHI AKIRA
Department of Ecological Engineering, Toyohashi University of Technology
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Kaiya Shinichi
Department of Ecological Engineering, Toyohashi University of Technology
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Futamata Hiroyuki
Department of Ecological Engineering, Toyohashi University of Technology
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Hiraishi A
Toyohashi Univ. Technol. Aichi Jpn
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Hiraishi Akira
Tokyo Research Laboratory Konishi Co. Ltd. :(present Address)institute Of Applied Microbiology Unive
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Kaiya Shinichi
Department Of Ecological Engineering Toyohashi University Of Technology
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Futamata Hiroyuki
Department Of Ecological Engineering Toyohashi University Of Technology:(present Office)department O
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Futamata Hiroyuki
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Futamata Hiroyuki
Department Of Material Science And Chemical Engineering Shizuoka University
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Futamata Hiroyuki
Department Of Ecological Engineering Toyohashi University Of Technology
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Hashizume Akiko
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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MIYAKODA HIDEKI
Sinto Environmental Measurement Centre, Shintokogyo Ltd.
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Futamata H
Department Of Ecological Engineering Toyohashi University Of Technology
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Miyakoda Hideki
Sinto Environmental Measurement Centre Shintokogyo Ltd.
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Hiraishi Akira
Department Of Biology Faculty Of Science Tokyo Metropolitan University
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